Advanced HPLC training (English language)

The training course will improve your knowledge of development and optimization of HPLC methods.
We aim at providing insight from a theoretical as well as from a practical perspective.
We use HPLC instrumentation from Agilent Technologies, but the course is not instrument-specific, and it will be relevant also for non-Agilent users.

• The use of chromatographic parameters (partition ratio, resolution, efficiency, symmetry) as a means of describing the chromatographic quality.
• Reverse-hase, normal-phase, and other forms of chromatography.
• Column selection: physical dimensions, choice of stationary phase, carbon load,…..
• Choosing your solvents.
• Isocratic and gradient analysis..
• Practical work with HPLC instrumentation.
• Optimization by changing column parameters.

The training course consists of a mixture of theory overview and practical work with HPLC instrumentation in our laboratory.

A basic understanding of chromatography is needed.
We expect the participant to have some prior experience with HPLC, either from a theoretical or from a practical point of view.
The course runs over two consecutive days, and is not instrument specific.

We use Agilent HPLC instrumentation for the illustration of construction principles and for a number of simpler practical excercises.

Mads (civ.ing. kemilinien) arbejder med applikationskemi, servicearbejde og undervisning.

Tore er kemiker (ph.d.) fra Københavns Universitet (kemi og fysik) med massespektrometri som speciale og med over 35 års undervisningserfaring i massespektrometri, chromatografi og analytisk kemi. Tore arbejder med undervisning, analysemetoder, salg og support samt med daglig ledelse af MSCi.