Advanced HPLC training (English language)
This training course is delivered in English
Training course overview
The training course will improve your knowledge of development and optimization of HPLC methods.
We aim at providing insight from a theoretical as well as from a practical perspective.
We use simple HPLC instrumentation from Agilent Technologies, but as such the course is not instrument-specific, and it will be relevant also for people not using instrumentation from this specific instrument provider.
Training course contents
- The use of chromatographic parameters (partition ratio, resolution, efficiency, symmetry) as a means of describing the chromatographic quality.
- Reverse-hase, normal-phase, and other fors of chromatography.
- Column selection: physical dimensions, choice of stationary phase, carbon load,…..
- Choosing your solvents.
- Isocratic and gradient analysis..
- Practical work with HPLC instrumentation.
- Optimization by changing column parameters.
Training methodology
The training course consists of a mixture of theory overview and practical work with HPLC instrumentation in our laboratory.
course prerequisites
A basic understanding of chromatography is needed.
We expect the participant to have some prior experience with HPLC, either from a theoretical or from a practical point of view.
The course runs over two consecutive days, and is not instrument specific.
Instrumentation
We use Agilent 1200 and 1100 HPLC instrumentation for the illustration of construction priciples and for a number of simpler practical excercies.
Undervisere
(Hold musen over for at se yderligere info)
(Hold musen over for at se yderligere info)
Priser
Kursustilmelding
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