Solid Phase Micro Extraction (SPME)

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If you are looking for a system with automated exchange of fibres, please look under MFX (Multi Fiber eXchange)

SPME – efficient and cheap technique

Solid Phase Micro Extraction (SPME) is a widely used technique for extraction and concentration of VOCs and SVOCs combined with GC/MS. SPME is especially useful in the food, flavour and beverage industries and for environmental monitoring. Unique to absorption techniques for GC, SPME is suited for both small and larger molecules, and the absorption may take place in either the headspace or liquid.
Our systems are optimized for performance and throughput in every step of the SPME process. Up to 128 samples placed in standard headspace vials can be analysed in one batch.

 

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Extraction

Sample agitation with stir bars accelerates the extraction process and improves throughput. Mechanical fibre stress is reduced, leading to longer average fibre life.

The PrepAhead function allows samples to be prepared in advance while the GC run is ongoing for improved throughput and system utilization.

In addition, samples can be heated or cooled during storage and extraction, which is highly useful, for example, for fresh food samples.

Desorption

The Cooled Injection System (CIS) is a PTV-type GC inlet, perfectly suited for SPME analysis. Programmed heating helps ensure that analytes are introduced to the GC without thermal breakdown. The narrow-bore inlet liner ensures highly efficient analyte transfer to the GC/MS for best possible detection limits and separation efficiency.

The patented Septum-Less Head (SLH greatly reduces mechanical stress on the SPME fibre, extending its life expectancy, and septum coring and background peaks from septum material are eliminated, leading to better limits of detection.

Efficient integrated control software

MAESTRO software supports a wide range of techniques including SPME. The PrepAhead function optimizes productivity and throughput: Samples are prepared just-in-time in parallel to the GC/MS run. Every sample is introduced exactly when the analysis system becomes ready for best possible system utilization and sample throughput. Priority samples can be inserted without halting the sequence.

MAESTRO can be operated independently or integrated with the Agilent Technologies ChemStation/MassHunter. Just one method and one sequence table runs the complete system from SPME to GC/MS analysis.

Visibility and overview

The MAESTRO Scheduler provides a graphical overview of SPME steps and the total analysis time for the entire batch of samples, making it easy to plan your laboratory work. The effect of any change to the method on productivity is immediately seen on the display, making it easier to optimize throughput.

Several user support functions are included: Context sensitive on-line help for easy set-up and log functions for traceability and troubleshooting.

On-fiber derivatization

Using the MPS, a derivatization reagent can be added to the fibre prior to or following the extraction step. This is a much-used accessory for the analysis of small aldehydes using the oxime formation by PFBHA reagent, but may be used for all reagents which react fast in the gas phase, e.g. MSTFA or diazomethane. These tasks are simply selected by mouse-click in the MAESTRO software.

Conditioning

Automated conditioning in the CIS or in the MPS bake-out station ensures that the fibre is always clean and ready for the next sample. Well conditioned SPME fibres provide analysis results with minimum RSD.

 

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