In contrast to GERSTEL’s automated SPE, where the final extract is eluted to an autosampler vial and the sample is injected from this, the GERSTEL SPExos is a true on-line SPE where the total eluate is transferred directly to LC. Compared with standard SPE, SPExos relies on much smaller cartridges with only 50 mg of sorbent, enabling a significant miniaturization of the entire process: much smaller sample amounts are needed to reach required detection limits and much less solvent is needed for analyte elution, reducing cost, and increasing method sensitivity.
The GERSTEL SPExos is inserted into the overall LC system between the GERSTEL MultiPurpose Sampler (MPS) and the HPLC system.
Online SPE can be combined with sample preparation steps performed by the MPS, including addition of standards, dilution, derivatization, and more. Following the MPS sample preparation steps, the sample is introduced to the SPExos system and from here directly to the LC system. As all sample preparation runs in the background while running the previous sample, this ensures minimum standard deviation on samples due to variations in the sample preparation time.
The complete system including MPS and SPExos is controlled by the GERSTEL Maestro Software, which again is 100% integrated with OpenLab/MassHunter.
User Selectable Cartridge exchange:
Sealed extraction system:
Cartridge inserted into the LC mobile phase for elution:
Small cartridges / small sorbent volume:
Easily adapted for multiple analysis methods:
Control through GERSTEL MAESTRO Software: